Effect of Insecticides and Plutella xylostella (Lepidoptera: Plutellidae) Genotype on a Predator and Parasitoid and Implications for the Evolution of Insecticide Resistance

In the laboratory and in cages in the greenhouse, we evaluated the toxicity of two insecticides (lambda-cyhalothrin and spinosad) on the parasitoid, Diadegma insulare (Cresson), and the predator, Coleomegilla maculate (DeGeer), both natural enemies of the diamondback moth, Plutella xylostella (L.). Lambda-cyhalothrin was very toxic to both natural enemies. Spinosad was less toxic to C. maculata adults and larvae, and slightly toxic to D. insulare. Both natural enemies suppressed P. xylostella populations in cages with 80% spinosad-treated and 20% nontreated plants; such suppression was not seen when lambda-cyhalothrin was used. Using broccoli, Brassica oleracea L. variety italica, a common host for P. xylostella, we also studied direct and indirect effects of both natural enemies in the presence and absence of the two insecticides and to different P. xylostella genotypes: resistant to the insecticide, susceptible, or heterozygous. Neither natural enemy could distinguish host genotype if P. xylostella were feeding on nontreated plants. They could also not distinguish between larvae feeding on spinosad-treated plants and nontreated plants, but D. insulare could distinguish between larvae feeding on lambda-cyhalothrin treated and nontreated plants. Our studies suggest that lambda-cyhalothrin has direct toxicity to these two natural enemies, can affect their host foraging and acceptance of P. xylostella and consequently would not be compatible in conserving these natural enemies in a program for suppression of P. xylostella. In contrast, our studies suggest that treatment with spinosad has much less effect on these natural enemies and would allow them to help suppress populations of P. xylostella. These findings are discussed in relation to the evolution of insecticide resistance and suppression of the pest population

Jet power extracted from ADAF and the applications to X-ray binaries and radio galaxy FR dichotomy

We calculate the jet power of the classical Blandford-Znajek(BZ) model and hybrid model developed by Meier based on the global solutions of advection dominated accretion flows (ADAFs) surrounding Kerr black holes. We find that the jet power of the hybrid model is larger than that of the pure BZ model. The jet power will dominate over the accretion power, and the objects will enter into "jet-power-dominated advective systems", when the accretion rate is less than a critical value mdot_c=Mdot_c/Mdot_Edd, where 3*10^-4 < mdot_c < 5*10^-3 is a function of black hole spin parameter. The accretion power will be dominant when mdot<mdot_c and the objects will enter into "accretion-power-dominated advective systems." This is roughly consistent with that constrained from the low/hard-state black hole X-ray binaries (e.g., Fender et al.). We calculate the maximal jet power as a function of black hole mass with the hybrid jet formation model, and find it can roughly reproduce the dividing line of the Ledlow-Owen relation for FR I/FR II dichotomy in the jet power-black hole(BH) mass plane (Q_jet-M_BH) if the dimensionless accretion rate mdot~0.01 and BH spin parameter j~0.9-0.99 are adopted. This accretion rate mdot~0.01 is consistent with that of the critical accretion rate for the accretion mode transition of a standard disk to an ADAF constrained from the state transition of X-ray binaries. Our results imply that most FR I galaxies may be in the ADAF accretion mode similar to the low/hard-state XRBs.Comment: 6 pages, 3 color figures, ApJ in press, edited with ApJ style and English is improved as suggested by Edito

On the origin of X-ray emission in some FR Is: ADAF or jet?

We investigate the X-ray origin in FR Is using the radio, submillimetre, optical, and {\em Chandra} X-ray data of a small sample consisting of eight FR I sources. These sources are very dim, with X-ray luminosities $L_{\rm X}/L_{\rm Edd} \sim 10^{-4}-10^{-8}$ ($L_{\rm X}$ is the X-ray luminosity between 2-10 keV). We try to fit the multiwaveband spectrum using a coupled accretion-jet model. In this model, the accretion flow is described by an advection-dominated accretion flow (ADAF) while in the innermost region of ADAF a fraction of accretion flow is transferred into the vertical direction and forms a jet. We find that X-ray emission in the source with the highest $L_{\rm X}$ ($\sim 1.8 \times 10^{-4}L_{\rm Edd}$) is from the ADAF. The results for the four sources with moderate $L_{\rm X}$ ($\sim$ several $\times 10^{-6}L_{\rm Edd}$) are complicated. Two are mainly from the ADAFs, one from the jet, and the other from the sum of the jet and ADAF. The X-ray emission in the three least luminous sources ($L_{\rm X} \lesssim 1.0\times 10^{-6}L_{\rm Edd}$) is dominated by the jet although for one source it can also be interpreted by the ADAF since the quality of X-ray data is low. We conclude that these results roughly support the predictions of Yuan & Cui (2005) where they predict that when the X-ray luminosity of the system is below a critical value, the X-radiation will not be dominated by the emission from the ADAF any longer, but by the jet. We also investigate the fuel supply in these sources. We find that the accretion rates in four sources among the five in which we can have good constraints to their accretion rates must be higher than the Bondi rates. This implies that other fuel supply, such as the gas released by the stellar population inside the Bondi radius, should be important.Comment: 31 pages, 8 figures, accepted for publication in Ap

Origin of the X-ray Emission in the Nuclei of FR Is

We investigate the X-ray origin in FRIs using the multi-waveband high resolution data of eight FR I sources, which have very low Eddington ratios. We fit their multi-waveband spectrum using a coupled accretion-jet model. We find that X-ray emission in the source with the highest L_X (~1.8*10^-4 L_Edd) is from the advection-dominated accretion flow (ADAF). Four sources with moderate L_X(~several*10^-6 L_Edd) are complicated. The X-ray emission of one FR I is from the jet, and the other three is from the sum of the jet and ADAF. The X-ray emission in the three least luminous sources (L_X<1.0*10^-6L_Edd) is dominated by the jet. These results roughly support the predictions of Yuan and Cui(2005) where they predict that when the X-ray luminosity of the system is below a critical value, the X-radiation will not be dominated by the emission from the ADAF any longer, but by the jet. We also find that the accretion rates in four sources must be higher than the Bondi rates, which implies that other fuel supply (e.g., stellar winds) inside the Bondi radius should be important.Comment: 6 pages. To published in Journal of Physics, in proceedings of "The Universe under the Microscope - Astrophysics at High Angular Resolution" (Bad Honnef, Germany, April 2008), eds. R. Schoedel, A. Eckart, S. Pfalzner, and E. Ro

Effect of Topological Defects on Buckling Behavior of Single-walled Carbon Nanotube

Molecular dynamic simulation method has been employed to consider the critical buckling force, pressure, and strain of pristine and defected single-walled carbon nanotube (SWCNT) under axial compression. Effects of length, radius, chirality, Stone–Wales (SW) defect, and single vacancy (SV) defect on buckling behavior of SWCNTs have been studied. Obtained results indicate that axial stability of SWCNT reduces significantly due to topological defects. Critical buckling strain is more susceptible to defects than critical buckling force. Both SW and SV defects decrease the buckling mode of SWCNT. Comparative approach of this study leads to more reliable design of nanostructures

Manipulating the 3D organization of the largest synthetic yeast chromosome

Whether synthetic genomes can power life has attracted broad interest in the synthetic biology field. Here, we report de novo synthesis of the largest eukaryotic chromosome thus far, synIV, a 1,454,621-bp yeast chromosome resulting from extensive genome streamlining and modification. We developed megachunk assembly combined with a hierarchical integration strategy, which significantly increased the accuracy and flexibility of synthetic chromosome construction. Besides the drastic sequence changes, we further manipulated the 3D structure of synIV to explore spatial gene regulation. Surprisingly, we found few gene expression changes, suggesting that positioning inside the yeast nucleoplasm plays a minor role in gene regulation. Lastly, we tethered synIV to the inner nuclear membrane via its hundreds of loxPsym sites and observed transcriptional repression of the entire chromosome, demonstrating chromosome-wide transcription manipulation without changing the DNA sequences. Our manipulation of the spatial structure of synIV sheds light on higher-order architectural design of the synthetic genomes. </p

Curcumin Enhances Neurogenesis and Cognition in Aged Rats: Implications for Transcriptional Interactions Related to Growth and Synaptic Plasticity

Background: Curcumin has been demonstrated to have many neuroprotective properties, including improvement of cognition in humans and neurogenesis in animals, yet the mechanism of such effects remains unclear. Methodology: We assessed behavioural performance and hippocampal cell proliferation in aged rats after 6- and 12-week curcumin-fortified diets. Curcumin enhanced non-spatial and spatial memory, as well as dentate gyrate cell proliferation as compared to control diet rats. We also investigated underlying mechanistic pathways that might link curcumin treatment to increased cognition and neurogenesis via exon array analysis of cortical and hippocampal mRNA transcription. The results revealed a transcriptional network interaction of genes involved in neurotransmission, neuronal development, signal transduction, and metabolism in response to the curcumin treatment. Conclusions: The results suggest a neurogenesis- and cognition-enhancing potential of prolonged curcumin treatment i

A meta-analysis of immune-cell fractions at high resolution reveals novel associations with common phenotypes and health outcomes

Background: Changes in cell-type composition of tissues are associated with a wide range of diseases and environmental risk factors and may be causally implicated in disease development and progression. However, these shifts in cell-type fractions are often of a low magnitude, or involve similar cell subtypes, making their reliable identification challenging. DNA methylation profiling in a tissue like blood is a promising approach to discover shifts in cell-type abundance, yet studies have only been performed at a relatively low cellular resolution and in isolation, limiting their power to detect shifts in tissue composition. Methods: Here we derive a DNA methylation reference matrix for 12 immune-cell types in human blood and extensively validate it with flow-cytometric count data and in whole-genome bisulfite sequencing data of sorted cells. Using this reference matrix, we perform a directional Stouffer and fixed effects meta-analysis comprising 23,053 blood samples from 22 different cohorts, to comprehensively map associations between the 12 immune-cell fractions and common phenotypes. In a separate cohort of 4386 blood samples, we assess associations between immune-cell fractions and health outcomes. Results: Our meta-analysis reveals many associations of cell-type fractions with age, sex, smoking and obesity, many of which we validate with single-cell RNA sequencing. We discover that naïve and regulatory T-cell subsets are higher in women compared to men, while the reverse is true for monocyte, natural killer, basophil, and eosinophil fractions. Decreased natural killer counts associated with smoking, obesity, and stress levels, while an increased count correlates with exercise and sleep. Analysis of health outcomes revealed that increased naïve CD4 + T-cell and N-cell fractions associated with a reduced risk of all-cause mortality independently of all major epidemiological risk factors and baseline co-morbidity. A machine learning predictor built only with immune-cell fractions achieved a C-index value for all-cause mortality of 0.69 (95%CI 0.67–0.72), which increased to 0.83 (0.80–0.86) upon inclusion of epidemiological risk factors and baseline co-morbidity. Conclusions: This work contributes an extensively validated high-resolution DNAm reference matrix for blood, which is made freely available, and uses it to generate a comprehensive map of associations between immune-cell fractions and common phenotypes, including health outcomes

Forebrain NR2B Overexpression Facilitating the Prefrontal Cortex Long-Term Potentiation and Enhancing Working Memory Function in Mice

Prefrontal cortex plays an important role in working memory, attention regulation and behavioral inhibition. Its functions are associated with NMDA receptors. However, there is little information regarding the roles of NMDA receptor NR2B subunit in prefrontal cortical synaptic plasticity and prefrontal cortex-related working memory. Whether the up-regulation of NR2B subunit influences prefrontal cortical synaptic plasticity and working memory is not yet clear. In the present study, we measured prefrontal cortical synaptic plasticity and working memory function in NR2B overexpressing transgenic mice. In vitro electrophysiological data showed that overexpression of NR2B specifically in the forebrain region resulted in enhancement of prefrontal cortical long-term potentiation (LTP) but did not alter long-term depression (LTD). The enhanced LTP was completely abolished by a NR2B subunit selective antagonist, Ro25-6981, indicating that overexpression of NR2B subunit is responsible for enhanced LTP. In addition, NR2B transgenic mice exhibited better performance in a set of working memory paradigms including delay no-match-to-place T-maze, working memory version of water maze and odor span task. Our study provides evidence that NR2B subunit of NMDA receptor in prefrontal cortex is critical for prefrontal cortex LTP and prefrontal cortex-related working memory